Comparative Genomic Hybridization (CGH) Microarray

Array based comparative genomic hybridization (array CGH) has emerged as a powerful tool for detecting gene copy number variants (CNVs) implicated in many disease states. ISENET performs CGH analysis of genomic DNA from human utilizing Agilent arrays with results available in 10-15 days.

It detects:

  • Genomic gains and losses 
    • Copy number variants (CNVs)
    • Duplications/deletions
    • Unbalanced translocations
    • Aneuploidies
  • >20% mosaicism (for example: cultures where >1 of 5 cells are trisomy 12)

It doesn’t detect:

  • Balanced translocations
  • Inversions
  • <20% culture mosaicism (for example: cultures where 1 of 5 cells is trisomy 12)
  • Chromosomal position of genomic gains
  • Loss of heterozygosity (LOH) / Absence of heterozygosity (AOH)

 It is used:

  • As a baseline genomic screen
    • To detect submicroscopic (<5Mb) abnormalities
    • To identify amplified or deleted genes of interest
  • In conjunction with g-banded karyotyping
    • To define translocation breakpoints
  • For research of genomic copy number change
    • To identify structural variation within populations or disease cohorts
    • To develop a cell line copy number variant profile

For more information or a quote, send an e.mail to info@isenet.it