The Cytogenetic Service should be used:
– when a cell line is established/derived;
– to monitor the genomic stability of a cell line (routine QC testing);
– at the beginning and conclusion of an experiment;
– for publication-quality karyotype;
– for cell banking;
– when a cell culture displays unusual growth properties.
References:1. Laurent, L. C. et al. Dynamic changes in the copy number of pluripotency and cell proliferation genes in human ESCs and iPSCs during reprogramming and time in culture. Cell Stem Cell 8, 106-118, doi: 10.1016/j.stem.2010.12.003 (2011). 2. Taapken, S. M. et al. Karyotypic abnormalities in human induced pluripotent stem cells and embryonic stem cells. Nat Biotechnol 29, 313-314, doi: 10.1038/nbt.1835(2011). 3. Blasco, M. A., Serrano, M. & Fernandez-Capetillo, O. Genomic instability in iPS: time for a break. EMBO J 30, 991-993, doi: 10.1038/emboj.2011.50 (2011). 4. Liang, Y. et al. The propensity for tumorigenesis in human induced pluripotent stem cells is related with genomic instability. Chin J Cancer 32, 205-212, doi:10.5732/cjc.012.10065 (2013). 5. Bai, Q. et al. Temporal Analysis of Genome Alterations Induced by Single-Cell Passaging in Human Embryonic Stem Cells. Stem Cells Dev, doi: 10.1089/scd.2014.0292(2014). ISENET routine genomic stability analysis are: 1. conventional karyotyping (Q-banding) on actively growing cells; 2. array Comparative Genomic Hybridization (aCGH) with a resolution down to 50kb (or less, depending on the array format);