Spectral-Karyotyping (SKY) is a technique based on the simultaneous visualization of all human chromosomes in different colors, therefore enables an accurate and easy detection of structural and numerical chromosomal aberrations. The technique combines Fourier spectroscopy, charged coupled device (CCD) imaging and optical microscope to measure simultaneously at all points of the sample emission spectra in the visible and near-infrared spectral range. This allows the use of multiple spectrally overlapping probes labeled with different dyes. SKY uses 24 chromosome specific probes generated by PCR from flow-sorted human chromosomes. Each chromosome is directly labeled with nucleotides conjugated to five different fluorescent dyes (FITC, Rhodamine, Texas Red, Cy5 and Cy5.5) or combinations thereof. All 24 human labeled chromosomes are combined together to create the SKY probe cocktail that is hybridized to the sample.
SKY can be used to define complex chromosomal rearrangements and identify marker chromosomes. Allows the detection of microscopic (>5-10 Mb) genomic abnormalities:
- Balanced and unbalanced translocations
ISENET performs SKY analysis using with optimization tools such as a review utility, advanced chromosome comparison and customized reports. We support human, rat and mouse karyotyping with spectral paint kits. When using other kits, ISENET supports all species karyotyping in addition to other spectral applications. ISENET guarantees:
- Automated analysis
- High resolution, full spectrum images
- Accurate, reliable, standardized and quick results
- Comprehensive data management