Q-BANDING KARYOTYPING

Technically, karyotype analysis investigates the number and structure of the chromosomes in one cell during metaphase stage of cell division. The chromosomes can be stained with Quinacrine Mustard (QFQ-banding: Q-Bands by Fluorescence using Quinacrine) or with Giemsa dye, resulting in a well defined banding pattern that enables the identification of each chromosome.

Q-banding karyotyping allows to obtain the following information:

  • overview of the whole genome;
  • species identification;
  • validation of normal diploid karyotypes;
  • numerical abnormalities (aneuploidies, i.e. trisomy, monosomy etc.);
  • structural abnormalities (with an overall resulution of 10-15Mb)
  • duplications and deletions
  • balanced/unbalanced translocations
  • inversions
  • isochromosomes
  • ring and marker chromosomes
  • Please note that karyotyping technique is not able to detected submicroscopic abnormalities and low-level mosaicism (<10%).

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